ParkinsonCanadaV1, Main, Exploration, bibRecord, 001169

Novel One-step Immunoassays to Quantify α-Synuclein

Identifieur interne : 001169 ( Main/Exploration ); précédent : 001168; suivant : 001170

Novel One-step Immunoassays to Quantify α-Synuclein

Auteurs : Michael Bidinosti ; Derya R. Shimshek ; Brit Mollenhauer [Allemagne] ; David Marcellin ; Tatjana Schweizer ; Gregor P. Lotz ; Michael G. Schlossmacher ; Andreas Weiss

Source :

The Journal of Biological Chemistry [ 0021-9258 ] ; 2012.

RBID : PMC:3460466

Abstract

Background: Robust assays for α-synuclein quantification are essential for Parkinson disease therapeutic development.

Results: TR-FRET immunoassays were validated for total and oligomeric α-synuclein and used to screen small molecules and kinases that regulate α-synuclein expression.

Conclusion: TR-FRET immunoassays are suitable for biomarker development and high-throughput screening.

Significance: This is the first platform for large-scale drug discovery and for neuronal pathway analysis of α-synuclein expression regulation.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3460466

DOI: 10.1074/jbc.M112.379792
PubMed: 22843695
PubMed Central: 3460466

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en"><p><bold>Background:</bold>
 Robust assays for α-synuclein quantification are essential for Parkinson disease therapeutic development.</p>
<p><bold>Results:</bold>
 TR-FRET immunoassays were validated for total and oligomeric α-synuclein and used to screen small molecules and kinases that regulate α-synuclein expression.</p>
<p><bold>Conclusion:</bold>
 TR-FRET immunoassays are suitable for biomarker development and high-throughput screening.</p>
<p><bold>Significance:</bold>
 This is the first platform for large-scale drug discovery and for neuronal pathway analysis of α-synuclein expression regulation.</p>
</div>
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La maladie de Parkinson au Canada (serveur d'exploration)

Novel One-step Immunoassays to Quantify α-Synuclein

Novel One-step Immunoassays to Quantify α-Synuclein

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Abstract

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